1kv5

Publication Abstract from PubMed
Triosephosphate isomerase (TIM) has a conserved salt bridge 20 A away from both the active site and the dimer interface. In this study, four salt bridge mutants of Trypanosoma brucei brucei TIM were characterized. The folding and stability of the mutants are impaired compared to the wild-type enzyme. This salt bridge is part of a hydrogen bonding network which tethers the C-terminal beta7-alpha7-beta8-alpha8 unit to the bulk of the protein. In the variants D227N, D227A, and R191S, this network is preserved, as can be deduced from the structure of the R191S variant. In the R191A variant, the side chain at position 191 cannot contribute to this network. Also the catalytic power of this variant is most affected.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure
1KV5 is a 2 chains structure of sequences from Trypanosoma brucei brucei. Full crystallographic information is available from OCA.

Reference
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